A sample of patient urine is collected for culture in a sterile container. At the time of collection, the urine sample has 25 cells/ml of E. coli. E. coli has a generation time of 30 minutes at room temperature. The sample is accidentally left at the nursing station desk for 8 hours before it is sent to the lab. A patient is considered to have a urinary tract infection (UTI) if there are more than 1000 cells/ml of urine. If a patient is positive for a UTI, appropriate antibiotics will be prescribed. 1. What is the number of E. coli present in the patient sample when it is brought to the lab? Does this patient actually have a UTI? Explain your answer. 2. Is antibiotic therapy appropriate in this case? How could this scenario lead to life threatening complications? How could this situation have been avoided?